Research Project: New Bioproducts for Advanced Biorefineries

Location: Bioenergy Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Biomass demineralization and pretreatment strategies to reduce inhibitor concentrations in itaconic acid fermentation by Aspergillus terreus - (Peer Reviewed Journal)

Developing a nonmembrane separation system to enable real-time recovery of acetone-butanol during fermentation - (Peer Reviewed Journal)
Okonkwo, C.C., Duduyemi, A., Ujor, V.C., Qureshi, N., Ezeji, T.C. 2024. Developing a nonmembrane separation system to enable real-time recovery of acetone-butanol during fermentation. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-024-13340-x.

Expanded genome and proteome reallocation in a novel, robust Bacillus coagulans capable of utilizing pentose and hexose sugars - (Peer Reviewed Journal)
Dolley, D., Ryu, S., Giannone, R.J., Edwards, J., Dien, B.S., Slininger, P.J., Trinh, C.T. 2024. Expanded genome and proteome reallocation in a novel, robust Bacillus coagulans capable of utilizing pentose and hexose sugars. mSystems. https://doi.org/10.1128/msystems.00952-24.

Process strategies for recovery of oil, sugars, and lipids from oilcane bagasse using natural deep eutectic solvents (NADES) - (Peer Reviewed Journal)
Raj, T., Dien, B.S., Singh, V. 2024. Process strategies for recovery of oil, sugars, and lipids from oilcane bagasse using natural deep eutectic solvents (NADES). Chemical Engineering Journal. https://doi.org/10.1016/j.cej.2024.152657.

Novel technologies for butyric acid fermentation: use of cellulosic biomass, rapid bioreactor, and efficient product recovery - (Peer Reviewed Journal)
Qureshi, N., Ashby, R.D., Nichols, N.N., Hector, R.E. 2024. Novel technologies for butyric acid fermentation: use of cellulosic biomass, rapid bioreactor, and efficient product recovery. Fermentation. https://doi.org/10.3390/fermentation10030142.

Pilot-scale processing of Miscanthus x giganteus for recovery of anthocyanins integrated with production of microbial lipids and lignin-rich residue - (Peer Reviewed Journal)
Banerjee, S., Dien, B.S., Eilts, K.K., Sacks, E.J., Singh, V. 2024. Pilot-scale processing of Miscanthus x giganteus for recovery of anthocyanins integrated with production of microbial lipids and lignin-rich residue. Chemical Engineering Journal. https://doi.org/10.1016/j.cej.2024.150117.

Rapid dilute sulfuric acid hydrolysis of soy flour to amino acids for microbial processes and biorefining - (Peer Reviewed Journal)
Slininger, P.J., Shea Andersh, M.A., Dien, B.S. 2023. Rapid dilute sulfuric acid hydrolysis of soy flour to amino acids for microbial processes and biorefining. Fermentation. https://doi.org/10.3390/fermentation9121028.

Determining mating type and ploidy in Rhodotorula toruloides and its effect on growth on sugars from lignocellulosic biomass - (Peer Reviewed Journal)
Dias Lopes, D., Dien, B.S., Hector, R.E., Singh, V., Thompson, S.R., Slininger, P.J., Boundy-Mills, K., Jagtap, S.S., Rao, C.V. 2023. Determining mating type and ploidy in Rhodotorula toruloides and its effect on growth on sugars from lignocellulosic biomass. Journal of Industrial Microbiology and Biotechnology. https://doi.org/10.1093/jimb/kuad040.

Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli - (Peer Reviewed Journal)
Liu, A., Machas, M., Mhatre, A., Hajinajaf, N., Sarnaik, A., Nichols, N.N., Frazer, S.E., Wang, X., Varman, A.M., Nielsen, D.R. 2023. Synergistic co-utilization of biomass-derived sugars enhances aromatic amino acid production by engineered Escherichia coli. Biotechnology and Bioengineering. https://doi.org/10.1002/bit.28585.

Can xylose be fermented to biofuel butanol in continuous long-term reactors: If not, what options are there? - (Peer Reviewed Journal)
Qureshi, N., Lin, X., Tao, S., Liu, S., Huang, H., Nichols, N.N. 2023. Can xylose be fermented to biofuel butanol in continuous long-term reactors: If not, what options are there? Energies. 16(13). Article 4945. https://doi.org/10.3390/en16134945.

Detoxification of high solid-liquid hydrothermal pretreated sugar cane bagasse by chromatographic adsorption for cellulosic ethanol production - (Peer Reviewed Journal)
Xian, X., Zheng, X., Huang, J., Qureshi, N., Li, B., Liu, J., Zeng, Y., Nichols, N.N., Lin, X. 2023. Detoxification of high solid-liquid hydrothermal pretreated sugar cane bagasse by chromatographic adsorption for cellulosic ethanol production. Industrial Crops and Products. (202): Article 117048. https://doi.org/10.1016/j.indcrop.2023.117048.

Lipid production by Rhodosporidium toruloides cultures grown on lignocellulose pretreated using pressurized ammonia ball milling - (Abstract Only)

Production of designer xylose-acetic acid enriched hydrolysate from bioenergy Sorghum, oilcane, and energycane bagasses - (Peer Reviewed Journal)
Cheng, M.H., Singh, S., Carr-Clennon, A.N., Dien, B.S., Singh, V. 2023. Production of designer xylose-acetic acid enriched hydrolysate from bioenergy Sorghum, oilcane, and energycane bagasses. Bioresource Technology. 380. Article 129104. https://doi.org/10.1016/j.biortech.2023.129104.

Constructing diploid Rhodosporidium toruloides yeast for production of single cell oil - (Abstract Only)
Dias Lopes, D., Dien, B.S., Singh, V., Jagtap, S., Rao, C., Hector, R.E. 2023. Constructing diploid Rhodosporidium toruloides yeast for production of single cell oil [abstract]. American Chemical Society Abstracts. Poster.

Lipid production by Rhodosporidium toruloides cultures grown on lignocellulose pretreated using pressurized ammonia ball milling - (Abstract Only)

A PCR based method for rapid identification of mating type in Rhodosporidium toruloides yeast - (Abstract Only)
Lopes, D., Dien, B.S., Singh, V., Jagtap, S., Rao, C., Hector, R.E. 2023. A PCR based method for rapid identification of mating type in Rhodosporidium toruloides yeast [abstract]. Symposium on Biomaterials, Fuels, and Chemicals . Poster No. M13/RM3-A.

From agricultural wastes to fermentation nutrients: A case study of 2,3-butanediol production - (Peer Reviewed Journal)
Okonkwo, C.C., Duduyemi, A., Ujor, V.C., Atiyeh, H.K., Iloba, I., Qureshi, N., Ezeji, T.C. 2022. From agricultural wastes to fermentation nutrients: A case study of 2,3-butanediol production. Fermentation. 9(1). Article 36. https://doi.org/10.3390/fermentation9010036.

A chemical-free pretreatment for biosynthesis of bioethanol and lipids from lignocellulosic biomass: An industrially relevant 2G biorefinery approach - (Peer Reviewed Journal)
Deshavath, N., Dien, B.S., Slininger, P.J., Jin, Y., Singh, V. 2022. A chemical-free pretreatment for biosynthesis of bioethanol and lipids from lignocellulosic biomass: An industrially relevant 2G biorefinery approach. Fermentation. 9(1). Article 5. https://doi.org/10.3390/fermentation9010005.

Production of biological pest control agents on hydrolysates of switchgrass - (Peer Reviewed Journal)
Slininger, P.J., Schoepke, A.R., Dien, B.S. 2022. Production of biological pest control agents on hydrolysates of switchgrass. Bioresource Technology Reports. 21. Article 101312. https://doi.org/10.1016/j.biteb.2022.101312.

The effects of sequential hydrothermal-mechanical refining pretreatment on cellulose structure changes and sugar recoveries - (Peer Reviewed Journal)
Cheng, M.H., Maitra, S., Carr Clennon, A.N., Appell, M., Dien, B.S., Singh, V. 2022. The effects of sequential hydrothermal-mechanical refining pretreatment on cellulose structure changes and sugar recoveries. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-022-03359-3.

Renewable energy production from novel and non-edible seed oil of Cordia dichotoma using nickel oxide nano catalyst - (Peer Reviewed Journal)
Rozina, Ahmad, M., Qureshi, N., Zafar, M., Ullah, S.A., Ul Abidin, S.Z. 2022. Renewable energy production from novel and non-edible seed oil of Cordia dichotoma using nickel oxide nano catalyst. Fuel. 332(1). Article 126123. https://doi.org/10.1016/j.fuel.2022.126123.

Butyric acid production by fermentation: Employing potential of the novel Clostridium tyrobutyricum strain NRRL 67062 - (Peer Reviewed Journal)
Qureshi, N., Liu, S., Saha, B.C. 2022. Butyric acid production by fermentation: Employing potential of the novel Clostridium tyrobutyricum strain NRRL 67062. Fermentation. 8(10). Article 491. https://doi.org/10.3390/fermentation8100491.

Copy number variants impact phenotype-genotype relationships for adaptation of industrial yeast Saccharomyces cerevisiae - (Peer Reviewed Journal)
Liu, Z., Huang, X. 2022. Copy number variants impact phenotype-genotype relationships for adaptation of industrial yeast Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-022-12137-0.

Solvent-free enzymatic esterification of free fatty acids with glycerol for biodiesel application: Optimized using the Taguchi experimental method - (Peer Reviewed Journal)
Singh, R., Dien, B.S., Singh, V. 2022. Solvent-free enzymatic esterification of free fatty acids with glycerol for biodiesel application: Optimized using the Taguchi experimental method. Journal of the American Oil Chemists' Society. 99(9):781-790. https://doi.org/10.1002/aocs.12633.

Itaconic acid production by Aspergillus terreus from glucose up to pilot scale and from corn stover and wheat straw hydrolysates using new manganese tolerant medium - (Peer Reviewed Journal)
Saha, B.C., Kennedy, G.J., Bowman, M.J., Qureshi, N., Nichols, N.N. 2022. Itaconic acid production by Aspergillus terreus from glucose up to pilot scale and from corn stover and wheat straw hydrolysates using new manganese tolerant medium. Biocatalysis and Agricultural Biotechnology. 43. Article 102418. https://doi.org/10.1016/j.bcab.2022.102418.

Near-complete genome sequence of Zygosaccharomyces rouxii NRRL Y-64007, a yeast capable of growing on lignocellulosic hydrolysates - (Rapid Release Publication)
Jagtap, S.S., Liu, J., Walukiewicz, H.E., Pangilinan, J., Lipzen, A., Ahrendt, S., Koriabine, M., Cobaugh, K., Salamov, A., Yoshinaga, Y., Ng, V., Daum, C., Grigoriev, I.V., Slininger, P.J., Dien, B.S., Jin, Y., Rao, C.V. 2022. Near-complete genome sequence of Zygosaccharomyces rouxii NRRL Y-64007, a yeast capable of growing on Lignocellulosic hydrolysates. Microbiology Resource Announcements. 11(5). Article e00050-22. https://doi.org/10.1128/mra.00050-22.

Cellulosic butanol biorefinery: production of biobutanol from high solid loadings of sweet sorghum bagasse - simultaneous saccharification, fermentation, and product recovery - (Peer Reviewed Journal)
Qureshi, N., Saha, B.C., Liu, S., Ezeji, T.C., Nichols, N.N. 2021. Cellulosic butanol biorefinery: production of biobutanol from high solid loadings of sweet sorghum bagasse - simultaneous saccharification, fermentation, and product recovery. Fermentation. 7(4): Article 310. https://doi.org/10.3390/fermentation7040310.

Exploring proteomes of robust Yarrowia lipolytica isolates cultivated in biomass hydrolysate reveals key processes impacting mixed sugar utilization, lipid accumulation, and degradation - (Peer Reviewed Journal)
Walker, C., Dien, B.S., Giannone, R., Slininger, P.J., Thompson, S.R., Trinh, C. 2021. Exploring proteomes of robust Yarrowia lipolytica isolates cultivated in biomass hydrolysate reveals key processes impacting mixed sugar utilization, lipid accumulation, and degradation. mSystems. 6(4). Article e00443-21. https://doi.org/10.1128/mSystems.00443-21.

Conversion of high-solids hydrothermally pretreated bioenergy sorghum to lipids and ethanol using yeast cultures - (Peer Reviewed Journal)
Cheng, M., Dien, B.S., Jin, Y.S., Thompson, S., Shin, J., Slininger, P.J., Qureshi, N., Singh, V. 2021. Conversion of high-solids hydrothermally pretreated bioenergy sorghum to lipids and ethanol using yeast cultures. ACS Sustainable Chemistry & Engineering. 9(25):8515–8525. https://doi.org/10.1021/acssuschemeng.1c01629.

Response surface methodology guided adsorption and recovery of free fatty acids from oil using resin - (Peer Reviewed Journal)
Singh, R., Dien, B.S., Singh, V. 2021. Response surface methodology guided adsorption and recovery of free fatty acids from oil using resin. Biofuels, Bioproducts, & Biorefining (Biofpr). 15(5): 1485-1495. https://doi.org/10.1002/bbb.2255.

Development and validation of time-domain 1H-NMR relaxometry correlation for high-throughput phenotyping method for lipid contents of lignocellulosic feedstocks - (Peer Reviewed Journal)
Maitra, S., Dien, B., Long, S.P., Singh, V. 2021. Development and validation of time-domain 1H-NMR relaxometry correlation for high-throughput phenotyping method for lipid contents of lignocellulosic feedstocks. Global Change Biology Bioenergy. 13(7): 1179-1190. https://doi.org/10.1111/gcbb.12841.