Location: National Peanut Research Laboratory
Project Number: 6044-21600-001-004-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 29, 2024
End Date: Jul 28, 2025
Objective:
1. To perform isolate collection from peanut field and production environments in peanut-producing counties in Georgia.
2. To perform whole-genome re-sequencing (WGRS) for >1,000 collected isolates for comparative and genome wide association (GWAS) analyses.
3. To identify genes associated with the regulation of aflatoxin production, morphological development, peanut pathogenicity, and fungicide resistance.
4. To investigate the evolution of identified genes and gene clusters associated with the selected traits to identify their sources and functions.
Approach:
The Cooperator will collaborate with ARS in conducting research to develop reduce aflatoxin contamination in United States peanuts.
Specifically, the cooperators will work with ARS in the following approaches: 1) Initial soil sampling for the microbial population survey will focus on established routines and locations used for physiological evaluation of plants in defined plots within peanut fields and also utilizing movable rainfall shelters and/or at the ARS Environmental Control Plot Facility at the National Peanut Research Laboratory. For microbiome analyses, a subsample of soil taken from the rainout plots at 0, 14, and 28 DAI will be used for genomic DNA isolation using a commercial soil DNA isolation kit. The results of the various research components will be published in refereed journals, trade magazines, or extension articles, presented at professional and grower meetings. Information from the research will be integrated into standard peanut processing procedures.
