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ARS Home » Research » Research Project #445159

Research Project: Expression of Rift Valley Fever Virus Proteins for Diagnostic Assay Development

Location: Biologics Development Module

Project Number: 3022-32000-026-010-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2023
End Date: Aug 31, 2025

Objective:
Advance the development of veterinary vaccine countermeasures to prevent, control, and respond to Rift Valley Fever incursions. Develop an ELISA based assay for detection of Rift Valley Fever Virus infection.

Approach:
Task 1. Cooperator designs constructs and expresses RVFV Gn and N proteins in baculovirus expression system. Task 2. Cooperator designs constructs and expresses RVFV Gn and N proteins in E. coli-based expression system. Task 3. ARS will evaluate expressed proteins for suitability in development of antibody-based diagnostic assay.