Location: Vegetable Crops Research
Project Number: 5090-21220-006-042-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2024
End Date: Jul 31, 2025
Objective:
To analyze intron splicing changes that occur in potato during infection with the late blight pathogen Phytophthora infestans. A specific protein from the pathogen called IPI-O1 is known to modulate intron splicing of specific genes in the plant. However, there are more unknown genes in potato that are also targeted. The purpose of this project is to identify differentially spliced genes in the presence or absence of IPI-O1 and develop methods to verify alternative intron splicing during P. infestans infection as well as test intron splicing under other stress conditions.
Approach:
Analyze RNA sequencing information to identify splicing junction sites that are differentially utilized when plants are exposed to IPI-O1 compared to plants without IPI-O1. The cooperator will carry out bioinformatic analysis of the RNAseq data to identify the intron splicing junctions and assist in developing polymerase chaine reaction primers that flank the introns, which will be used for verification by ARS.
