Location: Sunflower Improvement Research
Project Number: 3060-21220-034-035-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 15, 2024
End Date: Dec 31, 2025
Objective:
This proposal aims to mobilize white mold (WM) resistance into commercial common bean market classes. Investigators will:
1: Mobilization of WM resistance from P. coccineus and P. vulgaris in snap bean breeding lines.
2: Mobilization of white mold resistance in pinto bean
3: Mobilization of white mold resistance in black bean
4: Mine useful information, QTL and germplasm from the WM-MAGIC population.
Approach:
The aim of this project is to mobilize white mold (WM) resistance into commercial common bean market classes. These goals will be met by addressing four objectives.
Objective 1: A subset of 15 nested association (NAM) lines combining field and straw test resistance with good agronomic traits will be used to select resistant lines for release and use as parents in snap bean breeding. Six snap and two dry beans were used to create the Snap/Dry Bean (S/D) MAGIC population. Lines from the S/D MAGIC population were phenotyped for quality, photoperiod sensitivity, and dwarf lethal traits, and await GH straw (2024) and field (2025) screening for white mold resistance. S/D MAGIC lines with high productivity combined with white mold resistance will be identified and released.
Objective 2: Pinto beans are the #1 market class in US, but are highly susceptible to white mold. Early efforts introgressed resistance from exotic sources including race Mesoamerica, Andean, and P. coccineus sources. Populations resulting from these introgressions were subsequently used for QTL analyses including comparative mapping, meta-QTL analysis and fine mapping of major QTL in pinto bean. These QTL in resistant lines from the above populations will be used as donor parents to mobilize WM resistance in pinto bean with improved agronomic performance.
Objective 3: Black beans are the #2 market class in the US. Yet, advanced black bean lines were highly susceptible to WM The best black bean accession for combining field and straw test resistance is I9365-31 derived from a P. coccineus introgression. I9365-31 was introgressed into the black bean RIL population, R31 (Raven/I9365-31). Our plan is to use R31 RILs R31-24, R31-83, and others with the best field and straw test resistance combined with SNP haplotypes for narrowed QTL intervals to mobilize WM resistance in commercial black bean.
Objective 4: MAGIC populations are a tool to broaden the genetic base and facilitate applied research. The phenotyping and genotyping of the Durango WM-MAGIC population for pinto bean was completed. Novel QTL and previous QTL validated in this work were identified and utilized in genomic prediction models. The MAGIC population is also providing new pinto germplasm lines with increased resistance such as WMM-556 and WMM-750 with superior seed yield combined with resistance. Additional resistant and susceptible genotypes, reduced numbers of significant markers, and additional prediction models will be used to optimize prediction accuracy. SNP markers flanking useful QTL peaks identified from the predictive models will be developed for MAS.
