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Title: PROTECTION OF SWINE FROM FOOT-AND-MOUTH DIESASE WITH ONE DOSE OF AN ALL-D RETRO PEPTIDE

Author
item Nargi, Frances
item Kramer Jr, Edward
item MEZENCIO, J - FORMER PIADC EMPLOYEE
item Zamparo, Joann
item WHETSTONE, C - FORMER PIADC EMPLOYEE
item VAN REGENMORTEL, M-H - INST DE BIOLOGIA, FRANCE
item BRIAND, J - INST DE BIOLOGIA, FRANCE
item MULLER, S - INST DE BIOLOGIA, FRANCE
item Brown, Fred

Submitted to: Vaccine
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/8/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: Our previous work had shown that the D-amino acid peptide corresponding to the major immunogenic site of FMDV particles would elicit protective levels of neutralizing antibodies in guinea pigs. This study provides evidence that swine also can be protected against challenge by a single inoculation of the D-peptide.

Technical Abstract: Nine pigs were given a single inoculum of 100 ug of the all-D retro peptide corresponding to the immunodominant GH loop encompassing residues 141-159 of capsid protein VP1 of foot-and-mouth disease virus serotype A, sub-type 12. The peptide was conjugated to activated keyhole limpet haemocyanin and oil-adjuvanted before inoculation. The animals were challenged eleven weeks post-vaccination by exposing them to a pig which had been infected with the virus by inoculation. Two naive animals were included in the challenge study as controls. One of the vaccinated animals was completely unprotected and two developed very small lesions. None of the six remaining animals exhibited any clinical signs but two developed antibodies against non-structural proteins indicating that replication of the virus had occurred. No evidence of replication could be detected in the remaining four animals, either by rise in neutralizing antibody titre or by production of antibodies against non-structural proteins specific for virus replication.