Author
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Goth, Robert |
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ELLIS, P. - PHYTO DIAGNOSTICS COMPANY |
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DE VILLIERS, G. - PHYTO DIAGNOSTICS COMPANY |
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Goins, Ernest |
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WRIGHT, N. - PHYTO DIAGNOSTICS COMPANY |
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Ballesteros, Maria |
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 6/30/1999 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: In 1992, the Scottish Agricultural Science Agency detected a Carla virus (code name RLSV) with distinct serological and biological characteristics in the cv Red LaSoda. In 1993, a long filamentous rod-shaped virus particle was observed in a clone of the potato cv Red LaSoda during routine electron microscope testing of accessions in the Vancouver Collection of Virus-Free Potatoes being grown in California winter tests. Although the particle size of RLSV is similar to Potato Virus S and Potato Virus M, it is serologically distinct. A highly specific monoclonal antibody, 4E12, was developed for RSLV and used in TAS. In 1997 and 1998, 137 potato accessions in the U.S. National Varietal Collection maintained at Presque Isle, ME, were assayed for RLSV with TAS ELISA using the 4E12 Mab. Three cvs - High Plains, Platte, and Red LaSoda - tested positive for the virus. Additional systemic hosts for RLSV are Nicotiana benthimiana, N. megalosiphon, and N. occidentalis. |