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ARS Home » Southeast Area » Raleigh, North Carolina » Market Quality and Handling Research » Research » Publications at this Location » Publication #100749
Title: MICRO EXTRACTION AND CHARACTERIZATION OF SAPONINS IN PEANUT MEAL AND SOYBEAN FLOUR USING HPLC AND FAB MASS SPECTROMETRY

Author
item Singleton, John
item STIKELEATHER, LARRY - NC STATE UNIVERSITY
item HANEY, CAROL - NC STATE UNIVERSITY

Submitted to: Polish Phytochemical Society Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/8/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Saponins are very potent bioactive compounds found in many legumes and other plants and have been shown to exhibit health benefits such as cholesterol reduction, antioxidant activity, stress reduction, tumor suppression, and as a pain depressant. We have developed a low-cost extraction device for removing bioactive compounds from micro samples of peanut meal and soybean flour using pressure and temperature. This extraction procedure significantly reduces sample size, solvent consumption, and extraction time. Saponins were separated from the crude extracts by HPLC and characterized by Fast Atom Bombardment (FAB) mass spectrometry. Mass spectra investigation of extracted peanut samples has revealed the presence of four saponins and four saponins were extracted from soybean flour. FAB mass spectrometry provided information concerning molecular masses and aglycone composition.