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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #100896
Title: COMPARATIVE MAPPING BASED APPROACH TO CONSTRUCTION OF A GENOME MAP OF HEXAPLOID OAT

Author
item PORTYANKO, VLADIMIR - IOWA STATE UNIVERSITY
item Hoffman, David
item LEE, MICHAEL - IOWA STATE UNIVERSITY
item HOLLAND, JAMES - IOWA STATE UNIVERSITY

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/28/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Complete genetic maps have become an essential component of progress in crop improvement and basic genetics of cereal crops. The only published RFLP-map of the hexaploid (2n=6x=42) oat, Avena sativa L. genome has 38 linkage groups. The major objective of the current work is to develop a cultivated oat molecular map with the number of linkage groups corresponding to the basic number of chromosomes. Construction of an RFLP framework map based on an initial core set of 175 anchor probes mapped in other cereals is underway. The mapping population consists of 136 TAM-0-301 x Ogle F6:7 recombinant inbred lines. To date, a total of 114 marker loci, including 100 RFLP loci, 12 isozyme loci, and 2 morphological traits produced 18 linkage groups (LG) with coverage of 1087.9 cM. Multiple duplicated loci were detected by most of the probes mapped. Some sets of probes also displayed specific ortology. The two largest linkages, LG1 and LG2, together involved 57 marker loci at average density of 9.7 cM and correspond to RFLP linkage groups 6 and 5 of the Kanota x Ogle molecular map (O'Donoughue et al., 1995). Marker synteny between hexaploid oat and other related grasses were found for at least small genome fragments. A good colinearity in organization of maize chromosome 4 vs. LG1 and LG2 was revealed. RAPD and AFLP markers will be added to develop a complete hexaploid oat genome map.