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Title: ACTIVITY OF TRITERPENE GLYCOSIDES FROM THE ROOT BARK OF MUSSAENDA MACROPHYLLA AGAINST TWO ORAL PATHOGENS

Author
item KIM, NAM-CHEOL - U OF IL AT CHICAGO, IL
item CAI, LINING - U OF IL AT CHICAGO, IL
item Desjardins, Anne
item WU, CHRISTINE - U OF IL AT CHICAGO, IL
item KINGHORN, A - U OF IL AT CHICAGO, IL

Submitted to: Journal of Natural Products
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/23/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: In the Nepal Himalaya, we collected roots of a shrub called "dhobini" that are chewed by the local people to treat sore mouths, sore throats, and fever. Several novel compounds isolated from the roots were found to be inhibitory to a bacterium that causes periodontal disease. This ethnobotanical study has identified compounds that may be suitable for further development as therapeutic agents.

Technical Abstract: Four novel triterpene glycosides were isolated from the root bark of Mussaenda macrophylla. Their structures were determined as 3-O-beta-D-glucopyranosyl-28-O-alpha-L-rhamnopyranosyl-16alpha- hydroxy-23-dehydroxyprotobassic acid (1), 28-O-beta-D- glucopyranosyl-16alpha-hydroxy-23-dehydroxyprotobassic acid (2), 3-O-beta-D-glycopyranosyl-28-O-alpha-L-rhamnopyranoxyl-16alpha- hydroxyprotobassic acid (3), and 3-O-{[beta-D-glucopyranosyl (1-6)]-O-alpha-L-rhamnopyranosyl(1-2)-O-beta-D-glucopyranosyl}- O-beta-D-glucopyranosyl(1-3)-O-beta-D-glucopyranosylcycloart- 22,24-dien-27-oic acid (4). Four known triterpenes [3-O- acetyloleanolic acid (5), 3-O-acetyldaturadiol (6), rotundic acid (7), and 16alpha-hydroxyprotobassic acid (8)] were also isolated. The structures of 1-4 were determined by several spectroscopic techniques including 2D NMR methods. Compounds 1-6 showed inhibitory activity against a periodontopathic bacterium, Porphyromonas gingivalis but were inactive against the cariogenic organism, Streptococcus mutans.