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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #101235

Title: ALTERED METABOLISM AND GENE EXPRESSION IN CLUSTER ROOTS OF PHOSPHORUS- DEFICIENT WHITE LUPIN

Author
item ALLAN, DEBORAH - UNIVERSITY OF MINNESOTA
item Vance, Carroll

Submitted to: International Botanical Congress
Publication Type: Abstract Only
Publication Acceptance Date: 8/3/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Proteoid roots of phosphorus-deficient white lupin excrete phenomenal amounts of citrate and malate. Labeling studies have shown that a significant portion of the carbon excreted is fixed by phosphoenolpyruvate carboxylase (PEPC) in the roots. Proteoid root development is accompanied by: increased specific activity of malate dehydrogenase, citrate synthase, and PEPC; greater amounts of PEPC enzyme protein and mRNA; and increased acid phosphatase secretion from proteoid root segments. Presently, we are isolating and characterizing genes important in proteoid root development and the lupin P-deficiency response and evaluating their tissue and cellular expression patterns.