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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Publications at this Location » Publication #101871
Title: GENETIC VARIATION WITHIN A WORLDWIDE COLLECTION OF DIDYMELLA BRYONIAE USING AFLP ANALYSIS

Author
item KOTHERA, R. - CLEMSON UNIVERSITY
item KEINATH, A. - CLEMSON UNIVERISTY
item DEAN, R. - CLEMSON UNIVERISTY
item Farnham, Mark

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/12/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The fungal pathogen Didymella bryoniae is the causal agent of the foliar disease gummy stem blight on cucurbits. Little is known about the genetic diversity of this fungus. Amplified Fragment Length Polymorphism (AFLP) markers were used to elucidate genetic variation within D. bryoniae. A total of 56 isolates from five states (Florida, Georgia, Oklahoma, South Carolina, and Texas) and six countries (China, Greece, Israel, Sweden, The Netherlands, and the United States) were examined. Eight different AFLP primer-pair combinations yielded >51 polymorphic markers. Markers were scored by presence or absence. A portion of the primer-pair combinations was repeated to verify reproducibility. Cluster analysis will be used to determine genetic diversity within and among geographic regions.