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Title: IDENTIFICATION, SINGLE NUCLEOTIDE POLYMORPHISM (SNP) DISCOVERY, AND LINKAGE MAPPING OF BOVINE IMMUNE RESPONSE GENES

Author
item Chitko-Mckown, Carol
item Heaton, Michael - Mike
item Grosse, William
item Laegreid, William

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/21/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Concerns regarding the safety of the meat supply make it imperative for animal health researchers to identify genes which play a role in controlling infection in meat animals, and to identify superior alleles/haplotypes of these immune response genes which can be selected for in breeding systems. Using techniques such as PCR and differential display analysis we have identified a number of bovine cytokines expressed in response to infection with the enterohemorrhagic Escherichia coli O157:H7. Furthermore, we have sequenced these genes, identified single nucleotide polymorphisms (SNP) within them, and placed them on the MARC bovine linkage map. The polymorphic nature of these markers suggests that they will be useful for determining if variation in these genomic regions will influence the resistance to infectious diseases. We are currently using micro-array technology to continue our studies of gene expression in Escherichia coli O157:H7-stimulated cells. Results of these ongoing experiments will be presented.