Author
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Rendleman Jr, Jacob |
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Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 6/14/1999 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Short-chain amylose (av. dp 56-73), formed in aqueous solution by interaction of alpha-cyclodextrin with CGTase at 2-70 deg C, precipitated as highly insoluble, spherical granules (2-5 um in diameter). Granules formed at 2-25 deg C exhibited intense birefringence, indicating a high degree of structural order. X-ray diffraction patterns for amyloses prepared at 2 deg, 25 deg, and 50 deg C were B types; that for amylose prepared at 70 deg C was A type. Solubility in water at various temperature levels was studied and will be discussed. At 37 deg C, solubility was low (0.7-1.2 mg/ml) for all the amyloses. This fact would explain, in large part, the relatively low reactivity of the digestive enzyme alpha-amylase towards the granules. Although solubility increases greatly at temperatures above 100 deg C, efforts to produce films were unsuccessful. Studies pertaining to hygroscopicity, complexability, and size-exclusion chromatography of the new amyloses will also be discussed. |
