Author
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CHEN, YIWU - U OF ILL, URBANA |
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Nelson, Randall |
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Submitted to: Soybean Research World Conference Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 4/15/1999 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: China is the center of domestication of the soybean (Glycine max) but little information exists about genetic diversity in Chinese germplasm. The objectives of this research were to characterize and compare primitive germplasm accessions from four provinces in China: Zhejiang, Sichuan, Gansu and Hebei. Ten accessions from each province were characterized using RAPD fragments. CNS, an important U.S. ancestral line, was also included. A total of 241 fragments were scored of which 125 were polymorphic. Simple matching coefficients were calculated. Two hierarchical methods, Unweighted Paired Group Method using Arithmetic Averages (UPGMA) and Ward's minimum-variance method, produced five and four clusters, respectively. VARCLUS, a non-hierarchical procedure, produced six clusters. There was disagreement among the methods but each procedure identified clusters of accessions that originated from the same province. Based on all of the information, 6 major clusters containing 32 accessions were defined. Eight accessions from both Hebei and Zhejiang were divided between two clusters whereas accessions from Gansu (5) and Sichuan (4) were each placed in a single major cluster. The U.S. ancestral line CNS, which originated in a neighboring province, was placed in one of the Zhejiang clusters. The remaining nine accessions from Gansu, Sichuan and Zhejiang were placed in a single cluster by VARCLUS but were not grouped consistently by the two hierarchical methods. Because G. max is a domesticated species, determining genetic origin is generally uncertain, but among most of the G. max accessions in this research there is a strong relationship between origin and genetic similarity. |
