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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #104463
Title: CYTOLOGICAL AND FLUORESCENT IN SITU HYBRIDIZATION (FISH) SUTDIES ON ANTHER CULTURE-DERIVED HAPLOID AND ANEUPLOIDS OF DURUM WHEAT

Author
item ALTUNTEPE, MUNEVVER - CUKUROVA UNIV, TURKEY
item Peterson, Terrance
item Jauhar, Prem

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/1999
Publication Date: N/A
Citation: Altuntepe, M., Peterson, T.S., Jauhar, P.P. 1999. Cytological and fluorescent in situ hybridization (fish) sutdies on anther culture-derived haploid and aneuploids of durum wheat. Agronomy Abstracts, p. 83.

Interpretive Summary:

Technical Abstract: In an attempt to produce haploids of durum wheat, we produced several plantlets in Turkish cultivars by anther culture. Somatic and meiotic chromosomes were studied, using both conventional and fluorescent staining techniques. The haploids with 14 chromosomes were all albinos. Some albino regenerants had 28 chromosomes. Green plantlets generated from the same callus (probably originating from a single microspore) had 28 to 70 somatic chromosomes. These plants showed several abnor-malities, which increased with the level of polyploidy. An interesting feature was the preferential multiplication of B-genome chromosomes, which formed bivalents and multivalents (trivalents, quadrivalents and hexavalents). Both intragenomic and intergenomic multivalents were observed. Translocations involving the A- and B-genome chromosomes were also recorded. Chromosome breakages resulting in fragments and telocentrics, and dicentric chromosomes were observed. Chromosome multiplication and other aberrations induced during culture may form the bases of somaclonal and gametoclonal variations.