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ARS Home » Southeast Area » Florence, South Carolina » Coastal Plain Soil, Water and Plant Conservation Research » Research » Publications at this Location » Publication #104588
Title: ENHANCING COTTON FIBER STRENGTH WITH FORWARD CROSSING UTILIZING A TRANSGENIC DONOR

Author
item May Iii, Oscar
item WOFFORD, T. - MONSANTO - ST LOUIS, MO
item JOHN, M. - MONSANTO - MADISON, WI

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/30/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Exploitation of transgene x genetic background interactions is a possible method to enhance expression of inserted sequences. The goal of this work is to enhance fiber strength to effect increased efficiency of yarn manufacture. Transformation of 'Deltapine 50' (DP 50) was accomplished by Monsanto with particle bombardment to insert a GUS marker gene and a proprietary gene driven by a fiber specific promoter. Most GUS expressive DP 50 plants exhibited 30-70% enhanced fiber strength compared with the control, but inheritance was unstable. To overcome unstable inheritance, we examined outcross populations such as F2, F3, BC1F2 created by mating GUS expressive plants with various upland germplasm. Except for one F3 population, GUS expressive plants exhibited unstable inheritance of fiber strength, in that high strength plants produced GUS expressive progeny with a wide range of fiber strengths. The F3 population with stable inheritance eof enhanced fiber strength is currently being evaluated in F4 progeny rows