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Title: IDENTIFICATION OF AN IMMUNOGLOBULIN FC RECEPTOR BY PASTEURELLA HAEMOLYTICA A1

Author
item OSMUNDSON, RUTH - IOWA STATE UNIV.,AMES,IA.
item TABATABAI, LOUISA

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/4/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Bacterial Fc receptor expression is known to play a role in the pathogenesis of a variety of organisms. This study demonstrates the presence of an Fc receptor from a whole cell sonicate (WCS) preparation and the supernatant of a P. haemolytica A1 culture. A Western blot containing electroblotted proteins from P. haemolytica WCS and culture supernatant bound bovine Fc fragments which were detected with horseradish-conjugated IgG anti-bovine Fc fragments. Two distinct bands of 78,000 Da and 46,000 Da were present in the WCS preparation and culture supernatant. An additional less intense band of 107,000 Da was found in the supernatant. To more closely mimic in vivo conditions, P. haemolytica was also grown in iron replete and iron depleted media to determine whether Fc receptor expression is dependent on the presence of iron. While the other members of the Pasteurellacea family, Haemophilus and Actinobacillus, express Fc receptors, this is the first report of Fc receptor expression by any Pasteurella sp.