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ARS Home » Plains Area » Miles City, Montana » Livestock and Range Research Laboratory » Research » Publications at this Location » Publication #104893
Title: EVALUATION OF A PANEL OF MICROSATELLITE DNA MARKERS FOR USE IN INDIVIDUAL IDENTIFICATION OF BEEF CATTLE

Author
item STOCKBURGER, E - COLORADO STATE UNIVERSITY
item GREEN, R - COLORADO STATE UNIVERSITY
item WOOD, W - COLORADO STATE UNIVERSITY
item HOLM, T - PERKIN ELMER AGGEN
item Macneil, Michael
item SCHAFER, D - COLORADO STATE UNIVERSITY
item YEMM, R - COLORADO STATE UNIVERSITY
item BERG-RAMSEY, J - COLORADO STATE UNIVERSITY

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/1999
Publication Date: N/A
Citation: STOCKBURGER, E.M., GREEN, R.D., WOOD, W.O., HOLM, T., MACNEIL, M.D., SCHAFER, D.W., YEMM, R.S., BERG-RAMSEY, J. EVALUATION OF A PANEL OF MICROSATELLITE DNA MARKERS FOR USE IN INDIVIDUAL IDENTIFICATION OF BEEF CATTLE. JOURNAL OF ANIMAL SCIENCE SUPPLEMENT. 1999. v. 77(Suppl. 1). p. 100.

Interpretive Summary:

Technical Abstract: Recent progress in gene mapping has provided the beef cattle industry with microsatellite DNA markers useful for genotyping. Genotypes could provide the basis for unique individual animal identification (ID). Unique animal ID would help to provide needed carcass feedback information to breeders for use in genetic improvement programs. This project tested 9 markers suggested by the International Society of Animal Genetics (ISAG) to prove their efficiency in uniquely identifying individual animals. To test the markers, Hereford cattle (n=90) were sampled from three sources. The sample population was unique because most of the animals were inbred (Fx 0-55%), which provided a more stringent test of the markers. Blood was collected and DNA was extracted from each sample using conventional procedures. Each animal was genotyped with BM1824, SPS115, ETH3, ETH10, ETH225, TGLA122, TGLA53, TGLA126, and INRA23. Allelic frequencies for each marker were calculated post priori. Assuming Hardy-Weinberg equilibrium, these frequencies were used to determine the occurrence of the most common cumulative genotype over all 9 loci (6.4 x 10**-5). Allelic frequencies for the markers were not different among the 3 subpopulations (P>.10). Using the most common expected genotype frequency, the probability that any two random individuals in this population possess identical genotypes was no greater than 4.2 x 10**-9. The total number of possible genotypes with the 9 markers was 2.2 x 10**10. These data indicate that these 9 microsatellite markers are effective for individual animal ID and suggest that the cattle industry could pursue genotyping as a basis for an individual animal ID system.