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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #105677
Title: RAPID DETECTION OF LISTERIA MONOCYTOGENES IN FOOD AND FOOD ANIMALS (SWINE RESEARCH REPORT - 2000)

Author
item KANUGANTI, SREENIVAS - TUSKEGEE UNIV., ALABAMA
item WESLEY, IRENE
item REDDY, P - TUSKEGEE UNIV., ALABAMA

Submitted to: Swine Research Report
Publication Type: Proceedings
Publication Acceptance Date: 9/24/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The objective of this study was to streamline the detection and identification of Listeria monocytogenes (LM) in livestock and foods. Hog tonsil scrappings, hog tissues collected at necropsy, ground pork, and turkey washes were inoculated into UVM-1 (10%w/v) and after incubation (3 days) transferred to UVM-2. After 2 days, samples were plated to Palcam magar and incubated (48 hrs, 37 deg C). Characteristic LM colonies were verified using the multiplex PCR assay, which targets the 16S rRNA gene of Listeria and the hlyA gene unique to the species monocytogenes. To determine when LM achieved detectable levels during enrichment, template DNA was extracted directly from UVM-2 and Palcam agar and screened by multiplex PCR. When screened directly from UVM-2 by PCR, 34% (65 of 190) of ground pork samples and 19% (11 of 60) of turkey washes were positive for LM. In contrast, by multiplex PCR screening of colonies from Palcam agar, 39% (75 of 190) of ground pork and 29% (18 of 60) of turkey wash samples were positive. Future studies will focus on improved recovery from UVM-2 with immunomagnetic beads. LM positive isolates (n=33) of ground pork were serotyped and assigned to type 4 (72%) and type 1 (14%). 14% of isolates were neither serotype 1 nor 4. For live hogs, out of 150 samples each of carcasses, tonsils, and ileocecal lymph nodes tested, LM was detected once from tonsils and nodes by multiplex PCR. In contrast, LM was found on ~30% of ground pork produced from that same day from the packing plant.