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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #105887
Title: COMPARISON OF A MULTIPLEX AND 5' NUCLEASE PCR ASSAYS FOR THE RAPID DETECTION OF PATHOGENIC YERSINIA ENTEROCOLITICA IN SWINE AND PORK PRODUCTS (POSTER PRESENTATION)

Author
item BOYAPALLE, SANDHYA - TUSKEGEE UNIV., ALABAMA
item KANUGANTI, SREENIVAS - TUSKEGEE UNIV., ALABAMA
item Wesley, Irene
item REDDY, P - TUSKEGEE UNIV., ALABAMA

Submitted to: American Society for Microbiology Branch Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Pigs are the only animal reservoir of human pathogenic strains of Yersinia enterocolitica (YE). Our objective was to compare bacteriological culture method with PCR based protocols (multiplex PCR and TaqMan 5' nuclease PCR) for the rapid detection of pathogenic YE in market weight hogs and pork products. YE prevalence was compared in hog specimens collected before (fecal, tonsil scrapings) and after slaughter (lymph nodes, tonsils, cecal and rectal contents). By bacteriological culture method, YE was not detected in any hog tissues. By multiplex PCR, YE was detected in tonsil scrapings (2%), and tonsils (6%). The highest prevalence of YE was in ileocecal lymph nodes both by multiplex PCR and TaqMan assay. TaqMan assay detected YE in ~45% of all hog tissues. Chitterlings and ground pork were screened for YE. By culture method, YE was detected in chitterlings (10%), but not in ground pork. YE was identified in chitterlings (51%) and ground dpork (40%) by multiplex PCR and TaqMan assay, 85% and 80% respectively. The results suggest 1) YE was more frequently detected in chitterlings and ground pork than in freshly slaughtered hogs, indicating post slaughter contamination of pork products; and 2) the TaqMan assay is several-fold more sensitive than multiplex PCR and culture methods.