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Title: THE USE OF SSR MARKERS TO ASSOCIATE PRIMARY TRISOMICS WITH LINKAGE GROUPS IN SOYBEAN

Author
item Cregan, Perry
item XU, S. - UNIVERSITY OF ILLINOIS
item KOLLIPARA, K. - UNIVERSITY OF ILLINOIS
item SINGH, S. - UNIVERSITY OF ILLINOIS
item HYMOWITZ, T. - UNIVERSITY OF ILLINOIS

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/5/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary: A trisomic individual is one that has a normal set of chromosome pairs plus a third copy of one chromosome. Humans with Down's Syndrome are trisomic for chromosome 21. Trisomic plants have been studied for many years in corn and tomato. Any gene that is located on the trisomic chromosome will be inherited in a an unusual fashion, that is, the rules established by Gregor Mendel will not apply. Thus, trisomic plants can be used to associate a particular gene or DNA marker with a particular trisomic chromosome. In soybean, a set of trisomic individuals has finally been completed. Each member of the set of 20 soybean trisomics is trisomic for a different chromosome. A new genetic map of the soybean was recently developed that has about 1400 DNA markers or landmarks that are divided into 20 groups referred to as linkage groups. The linkage groups are assumed to correspond to the 20 soybean chromosomes. We report a procedure e to use the simple sequence repeat DNA markers on the new map of the soybean to rapidly detect deviations from normal inheritance patterns in genetic populations developed from the soybean trisomics. This permits the easy association of the 20 linkage groups with the 20 soybean chromosomes. This procedure will be used by soybean geneticists to associate each of the 20 newly defined soybean linkage groups with their respective soybean chromosome.

Technical Abstract: Primary trisomics are a classical cytological tool to associate genes and linkage groups (LG) with their respective chromosomes. A complete set of 20 primary trisomics has recently been established in soybean [Glycine max (L.) Merr.]. Likewise, a linkage map of soybean, with 20 consensus LGs has recently been defined. It was the objective of the work reported here e to define a procedure to quickly associate each of the 20 consensus LGs with their respective chromosome. Because simple sequence repeat (SSR) markers map to defined single positions in the soybean genome, the association of a SSR locus with a chromosome will provide an unambiguous association of LG and chromosome. To examine this application of SSR markers, populations of F2 plants were developed from F1 hybrids trisomic for chromosomes 5 and 13. Polymorphic SSR markers from different LGs were tested on a subset of 20 plants from each population to identify markers that showed trisomic inheritance. Selected markers were further examined in the complete population of F2 plants. Chromosomes 5 and 13 were associated LGs A1 and F, respectively. The development of this rapid cytogenetic/molecular procedure will be useful in associating each of the 20 newly defined soybean LGs with their respective soybean chromosome.