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ARS Home » Research » Publications at this Location » Publication #106480
Title: GENETIC ANALYSIS OF XANTHOMONAS AXONOPODIS PV CITRI AND X. A. PV. AURANTIFOLII USING RANDOM AMPLIFIED POLYMORPHIC DNA FRAGMENTS

Author
item MYUNG, INN - RURAL DEV. AGENCY, KOREA
item CHO, YONG - SEOUL NATIONAL UNIVERSITY
item Hartung, John

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2000
Publication Date: 2/1/2001
Citation: Myung, I.S., Cho, Y.S., Hartung, J.S. 2001. Genetic analysis of xanthomonas axonopodis pv citri and x. a. pv. aurantifolii using random amplified polymorphic dna fragments. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Genetic diversity among 57 strains of Xanthomonas axonopodis pv. citri and 18 strains of X. a. pv. aurantifolii, associated with different forms of citrus bacterial canker disease was investigated using Random Amplified Polymorphic DNA (RAPD). One hundred and seventy-five different RAPD fragments were consistently produced by polymerase chain reaction (PCR) amplification with 19 different 10-mer primers. Genetic distances among the bacterial strains were calculated by Nei's algorithm and a phenetic tree was derived with the Unweighted Pair Group Method with Arithmetic Mean Analysis (UPGAM). Three different patterns, A - B/C/D, A/B/C/D, and A - B/D - C were observed in RAPD profiles, depending on primer used for PCR amplifications. The cluster analysis of RAPD data revealed phenetic clusters similar to those obtained previously from RFLP analyses of genomic DNA and plasmid DNA fingerprints. The dendrogram derived from combined RAPD data showed that the two pathovars were clearly differentiated at a level of about 24% dissimilarity. X. a. pv. citri strains were further separated into two groups. Forty-nine strains of X. a. pv. citri formed a single group, whereas the other strains that included 5 previously reported as variants formed a second group. Strains of X. a. pv. aurantifolii were also separated into two distinct groups; one contained pathotypes B/D and the other contained only pathotype C. This study shows that strains of X. a. pv. citri and X. a. pv. aurantifolii exhibit genetic diversity detectable by RAPD analysis, and that cluster analysis of RAPD fragments can be used both to distinguish between strains of each pathovar and to determine relatedness between them.