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Title: LINKAGE MAPPING OF DNA MARKERS GENERATED WITH SPECIFIC AND NON-SPECIFIC GENE PRIMERS IN SOYBEAN

Author
item SOLIMAN, K - ALABAMA A&M
item PALADUGU, M - ALABAMA A&M
item Devine, Thomas

Submitted to: Biologia Plantarum
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/10/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary: Genes are the entities that control inheritance. In plants, the genes are located in linear order along thread-like structures called chromosomes. The location of the genes on the chromosome determines whether they tend to be co-inherited (as when they are located close together on the same chromosome) or inherited independently (as when the genes are far apart on the same chromosome or on different chromosomes). In this study, 30 molecular marker genes were found to be linked and distributed between five linkage groups and 13 marker genes were found to be independently inherited. This information is useful in constructing comprehensive genetic map of soybean chromosomes. Such information will make breeding of multiple pest resistant cultivars with high oil and protein content much more efficient. This information will be used by soybean breeders in developing improved soybean cultivars, thereby making soybean products available to consumers at reasonable prices and maintaining the competitiveness of U.S. soybean production in world market.

Technical Abstract: Polymerase chain reaction (PCR) has been extensively in the construction of linkage maps for many cultivated crops including soybean, [Glycine max (L.) Merr.]. In this study, four sets of oligonucleotide primer pairs of known genes (pearl millet Adh 1, nodule specific proline-rich protein, Drosophila homeobox, heat shock protein), several different combinations from kits A, D, E, and J (Operon Technologies) of arbitrary primers and five primers pairs of soybean simple sequence repeats of varying length (Satt 9, Satt 20, Satt 42, Satt 64, & Satt 30) were utilized in PCR to identify molecular markers which were the used to construct a genetic linkage map. DNA for the PCR reactions was isolated from 65 recombinant inbred soybean lines resulting from crossing PI 290,136 and BARC-2 (Rj4), followed by self-pollination for seven generations without selection. Mapmaker 3.0, a computer package, was used for construction of the linkage map. A total of 43 polymorphic markers were identified; 30 markers were linked and distributed among 5 linkage groups while 13 markers were unlinked. Arbitrary primers revealed more polymorphisms than specific primers. A combination of arbitrary primers A5 & A18 revealed the maximum number of polymorphic bands. Five observed linkage groups can be expanded in future soybean research by using additional markers.