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Title: DETECTION OF GENETIC VARIATION AMONG SCLEROTINIA MINOR ISOLATES USING RAPD-PCR AND DNA-DNA REASSOCIATION ANALYSIS

Author
item Chamberlin, Kelly
item Melouk, Hassan

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2000
Publication Date: 7/1/2000
Citation: Chenault, K.D., Melouk, H.A. 2000. Detection of genetic variation among Sclerotinia minor isolates using RAPD-PCR and DNA-DNA reassociation analysis. Phytopathology. Available: http://www.apsnet.org/meetings/2000/search/p00ma096.htm.

Interpretive Summary:

Technical Abstract: Sclerotinia minor Jagger, which is a soilborne fungus that infects peanut and causes Sclerotinia blight, a disease that results in significant yield losses. Two techniques, RAPD-PCR and DNA-DNA reassociation analysis, were used to identify genetic variation among 13 Oklahoma isolates of S. minor. DNA polymorphisms were detected among different isolates using 20 oligonucleotide primers, and distinct groups of isolates were identified. The DNA-DNA reassociation values further determined the levels of relatedness among the S. minor isolates and supported the existence of the groups identified by RAPD-PCR profiles. These results are indicative of the genetic variation that can exist among S. minor isolates in a small geographic region.