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Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 5/10/2000 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: The soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pest of soybean and causes an estimated loss of 213 million bushels of soybean worth $1.5 billion each in the U.S.A. We are studying the resistance response of soybean to SCN using differential display and array techniques. A cDNA library was constructed from cv. Peking mRNA two days after invasion of roots by SCN race 3. Over 1000 ESTs were sequenced by one-pass sequencing the 5' end of randomly picked clones. The identities of numerous clones were determined through BLAST searches, including genes involved in secondary metabolism, signaling, and the defense response. Several genes involved in phenylpropanoid synthesis were found including phenylalanine ammonia lyase, chalcone synthase, cinnamyl alcohol dehydrogenase and 4-coumarate coA ligase. In addition, peroxidases, protein kinases and transcription factors were identified. Inserts from the cDNAs were PCR amplified and analyzed by gel electrophoresis. Sizes of the inserts ranged from 400 bp to over 3.2 kb. Our database includes clones from differential display experiments that identified SCN-induced soybean genes and a set of 96 clones was added from a cDNA library made from cotyledons to serve as controls that are probably not induced by SCN invasion. Inserts of several clones in our database are completely sequenced and contain complete reading frames: these are from genes that were available in this or other USDA laboratories. The database containing clone information is searchable by clone address, identity and size. The database can be viewed by collaborators at http://bldg6.arsusda.gov/benlab. Benjamin F. Matthews 301-504-5730, bmatthew@asrr.arsusda.gov |
