Author
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SLODKI, MOREY - NCAUR/USDA/ARS (RETIRED) |
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Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 7/14/2000 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Phosphomannan Y-2448 and related extracellular poly(phosphodiesters) are produced in high yields by a small group of related yeasts during aerobic growth on media containing excess D-glucose and orthophosphate. Although the highly phosphorylated biopolymers give viscous and rapidly shear-thinning solutions, sensitivity of the viscosity to salts makes them commercially unsuitable. Cleavage of hemiacetal phosphate bonds by mild acid hydrolysis, however, affords a 9:1 mix of pentasaccharide and high-molecular-weight (core) monoester products, which are useful as ligands for interactions with mammalian mannose 6-phosphate, insulin- like growth factor II and peripheral lymphocyte homing receptors. Recent work at the Carlsberg Laboratory reveals much about the product and macro-molecular structures [L.A.S. Parolis et al., Carbohydr. Res. 293:101(1996); 309:77(1998)]. A core with doubly-branched, doubly- phosphorylated, six-residue repeats is proposed, with each of the branches bearing at least ten oligosaccharide phosphodiesters. |
