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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #111742
Title: MUTANT MAPPING IN THE MISSOURI MAIZE PROJECT

Author
item CARSON, C - UNIV OF MISSOURI-COLUMBIA
item MELIA-HANCOCK, S - UNIV OF MISSOURI-COLUMBIA
item COE JR, EDWARD

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/17/2000
Publication Date: 3/17/2000
Citation: CARSON, C., MELIA-HANCOCK, S., COE JR, E.H. MUTANT MAPPING IN THE MISSOURI MAIZE PROJECT. MAIZE GENETICS CONFERENCE. 2000. ABSTRACT P. 82.

Interpretive Summary:

Technical Abstract: The mutants of maize are a valuable resource. They provide a rich source of variation accessible for identifying gene function. Our goal is to determine the bin locations of the many relatively uncharacterized mutants at the Maize Genetics COOP and from internal resources at the University of Missouri. While most of these mutants (70%) are unplaced and require a larger set of markers per mutant to identify map location, we are currentl working with a set of mutants (~500) that were created and placed to chromosome arms using B-A translocations by Dr. M.G. Neuffer at the University of Missouri. F1 materials were produced from crosses between mutant stocks and up to four inbred lines: A619, A632, B73, and Mo17. F2 materials have been grown out and we have collected samples from 67% of them. We are presently mapping mutants to SSR-loci detected by PCR and SFR- agarose electrophoresis. Stable, crude extracts of sample tissues (endosperm, seedling, maturing adult leaves, and immature ear buds) are prepared by a simple, inexpensive method that is effective and safe. Dilutions are used directly in PCR. Our strategy involves first determining the best polymorphic markers from pools of segregating F2 samples. Then we analyze 18-24 F2 homozygous individuals from each mutant family with as many arm-specific polymorphic markers as necessary (6-12 polymorphic markers per mutant family, depending on the availability of markers per chromosome arm). We are optimizing the process to maximize our rate of mutant mapping. We will provide a report descripting our method, progress, successful mutant mappings, and future strategies.