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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #113768
Title: PROPAGATION OF THE SOYBEAN CYST NEMATODE ON HAIRY ROOTS AND EXPRESSION OF RESISTANCE IN TRANSGENIC ROOTS

Author
item CHO, HYEON-JE - U OF ILL, URBANA
item Noel, Gregory
item FARRAND, STEPHEN - U OF ILL, URBANA
item EGGETT, THERESE - U OF ILL, URBANA
item WIDHOLM, JACK - U OF ILL, URBANA

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 6/25/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Hairy roots of soybean were induced by Agrobacterium rhizogenes on Heterodera glycines-susceptible Lee 68, Mandarin, Maple Arrow and Williams 82, and H. glycines-resistant Cartter, Fayette, Hartwig, Jack, Peking and PI 437654 at rates of 54-95 % on selecting a medium containing 200 ug/ml kanamycin and 500 ug/ml carbenicillin. Eight weeks after inoculation with H. glycines eggs, the number of cysts formed on hairy roots of the resistant cultivars was 0 to 2, whereas the number on the susceptible cultivars was 0 to 175, indicating that the resistance phenotype was preserved in transgenic hairy roots. Several candidate genes were introduced into Williams 82, for evaluation of SCN-resistance imparted by these genes. The expression of transgenes was confirmed by polymerase chain reaction, Southern, Northern, and Western blots. The resistance of these candidate genes to H. glycines is now being tested.