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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #115615
Title: COMPREHENSIVE GENETIC, PHYSICAL, AND DATABASE RESOURCES

Author
item COE JR, EDWARD
item CONE, K - UNIV OF MISSOURI-COLUMBIA
item GARDINER, J - UNIV OF MISSOURI-COLUMBIA

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/18/2000
Publication Date: 3/18/2000
Citation: COE JR, E.H., CONE, K., GARDINER, J. COMPREHENSIVE GENETIC, PHYSICAL, AND DATABASE RESOURCES. MAIZE GENETICS CONFERENCE. 2000. ABSTRACT P. 6.

Interpretive Summary:

Technical Abstract: Integrated genetic and physical maps; resources and tools for mapping; data management, analysis, and compilation; and effective dissemination of information to users are targets of the Missouri Maize Project (MMP). Restriction fragment length polymorphisms (RFLPs) and new SSR markers are placed on the high-resolution Intermated B73/Mo17 recombinant inbred panel (IBM). Mapped markers will be anchored on three BAC libraries: 5X HindIII 5X EcoRI, and 5X MboI. Goals are 1,000 RFLPs and 2,000 SSRs from maize mapped and anchored, plus 1,000 RFLPs from sorghum. Information on all publicly available SSR markers is in the Maize Genome Database (MaizeDB). Many of the loci are based on cDNA clones. Fingerprinting of the BAC libraries is being initiated. The progress and relative efficacy of anchoring, fingerprinting, and other assembling methods will be assessed in stages. Progress is being made toward construction of a panel of radiation hybrids of maize genomes in Chinese hamster ovary cells. Procedures have been developed for high-throughput mapping of mutants for visible traits, and mapping is progressing. Characterization and systematization of mutant and trait descriptions is under development toward enhancement in parallel with mapping and with developing programs in structural and functional genomics. Within MMP data undergo self-verifying entries at the LabDB level. The data from MMP and other projects and publications are ported into MaizeDB and are disseminated in tables, front-ends, and search utilities, integrated and linked with all publicly available data at http://www.agron.missouri.edu/. The MaizeDB encyclopedia indexes and cross-indexes knowledge about genetic differences; genetic change; gene expression; growth and development; physiology; and pests and stresses.