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ARS Home » Research » Publications at this Location » Publication #115636
Title: PHOTOSYNTHESIS AND RESPIRATION: CHARACTERIZATION OF THE BIOPHYSICAL PROPERTIES OF CYTOCHROME C OVERPRODUCED IN ESCHERICHIA COLI AND ITS FUNCTION IN CYANOBACTERIUM SYNECHOCYSTIS 6803

Author
item CHO, YOON - PLANT BIOLOGY UOFI URBANA
item WHITMARSH, CLIFFORD

Submitted to: Plant Physiology Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 7/19/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cytochrome cm is a novel c-type cytochrome which has been identified by genetic analysis in the cyanobacterium Synechocystis 6803. It has been suggested that cytochrome cm (1) acts as an alternative electron carrier to replace cytochrome c6 and plastocyanin in photosynthetic electron transport reactions or, (2) serves as a redox intermediate between soluble electron carriers and the cytochrome oxidase. To obtain a sufficient amount of cytochrome cm protein for the characterization of its biophysical, structural and functional properties, we have overproduced the cytochrome in E. coli cells. Several biophysical properties of the overproduced protein have been determined including the abosorbance spectrum, the extinction coefficient, the isoelectric point, and the redox midpoint potential. The mutant strain of Synechocystis in which a cytochrome c6 gene is replaced with a cytochrome cm gene has been created. This work opens the way for determining the three-dimensional structure of cytochrome cm and for the discovering its function in cyanobacteria.