Author
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Curren, Meredith |
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King, Jerry |
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Submitted to: Pittsburgh Conference
Publication Type: Abstract Only Publication Acceptance Date: 10/11/2000 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Current methodologies for the removal of toxicants from animal products require multiple steps for sample clean-up and extraction due to the complex nature of meat matrices. In addition, these procedures usually require large amounts of toxic, organic solvents that have high requisition and disposal costs. In this study, subcritical or "hot" water was utilized das an environmentally benign solvent for the ASE of three triazine pesticides from beef kidney. The ASE method is used in conjunction with matrix solid phase dispersion (MSPD) to retain the matrix components. Using these techniques, sample clean-up and analyte extraction are performed in a single step to minimize loss of the analyte, improve extraction efficiency, and increase sample throughput. The advantages of utilizing subcritical water are its non-toxic nature, low cost, and that it can be readily obtained and disposed of. In addition, its solvent properties can be varied as a function of temperature during a pressurized extraction. The removal of the pesticides from beef kidney is facilitated with the addition of ethanol to the water. The addition of this modifier does not compromise the environmental and health benefits of the extraction agent. For extractions, beef kidney contaminated with atrazine, cyanazine, and simazine is dispersed with a polymeric resin, followed by automated ASE at 100 degrees celsius and 50 atmospheres. The resultant aqueous extract from the ASE free of the particulate matter often associated with a meat matrix. The extracted analytes are then concentrated on a reverse-phased sorbent disk and are subsequently quantified by GC/MS. |
