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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #117117
Title: AN RFLP GENETIC LINKAGE JOINMAP DEVELOPED FROM FOUR F2.3 UPLAND COTTON POPULATIONS

Author
item Ulloa, Mauricio
item Meredith Jr, William
item SHAPPLEY, Z. - MONSANTO
item KAHLER, A. - BIOGENETICS SERVICES, INC

Submitted to: International Plant and Animal Genome IX Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/13/2001
Publication Date: 1/13/2001
Citation: ULLOA, M., MEREDITH JR, W.R., SHAPPLEY, Z.W., KAHLER, A.L. AN RFLP GENETIC LINKAGE JOINMAP DEVELOPED FROM FOUR F2.3 UPLAND COTTON POPULATIONS. INTERNATIONA PLANT AND ANIMAL GENOME IX CONFERENCE. 2001. p. 129

Interpretive Summary: Entering acceptance date, publication date and citation. 10/6/03

Technical Abstract: We have established the first RFLP joinmap of Gossypium hirsutum L. as a first step toward further understanding the structural genomics of Upland cotton, identifying homologous synteny regions with a set of cDNA-probes. The joinmap was amalgamated from four cotton populations. The first and the second genetic maps were previously reported (Ulloa and Meredith 2000; Shappley et al. 1998). The third genetic map comprises 83 loci mapped to 24 linkage groups, covering 830.1 centiMorgan (cM) or approximately 18% of the genome. The fourth genetic map comprises 56 loci mapped to 16 linkage groups, covering 520.4 cM or approximately 11% of the cotton genome. The genetic linkage joinmap from the above four populations comprises 283 loci, from 201 cDNA probe/enzyme (EcoRI and EcoRV) combinations and the locus for pollen color (P1), mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1502.6 cM or approximately 31% of the total recombinational length of the cotton genome. The joinmap provided further knowledge of ancestral synteny and gene order, and increased the potential to map genes for the improvement of the cotton crop. Research is ongoing for the identification of quantitative trait loci for agronomic, physiological, and fiber quality traits on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors.