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ARS Home » Research » Publications at this Location » Publication #117167
Title: PRODUCTION OF EXTRACELLULAR PHENOLIC ANTIOXIDANTS PROVIDES A BUFFER AGAINST LOW-LEVEL BUILDUP OF ROS IN PLANTS

Author
item Baker, Con
item Oneill, Nichole
item Deahl, Kenneth
item Roberts, Daniel
item Buyer, Jeffrey

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/22/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We have been investigating the role of ROS in the early events of plant/pathogen interactions. Potato and tobacco suspension cells were first washed and suspended in assay buffer, then placed on a shaking water bath and finally inoculated with virulent and avirulent bacterial pathogens. Within the first few hours there is a two-phased ROS burst. The efirst phase, which begins immediately after inoculation, occurs in both susceptible and resistant interactions. The second phase begins about 2 hr. after inoculation and only occurs in resistant interactions. The scavenging capability of these cells was monitored during this period using a technique that measures the rate of decrease of exogenously added hydrogen peroxide. This lead to the observation in control cells that antioxidant levels were building up and neutralizing the exogenously added hydrogen peroxide within 1 to 2 sec. The antioxidants appear to be small molecular weight phenolic compounds. Based on the spectrum from 210-350 n there are several different compounds involved depending on the age and type of plant cell. These compounds are currently being isolated and identified. It appears they do not accumulate in stressed cells because they react with the endogenous hydrogen peroxide as it is produced. Therefore due to the production of these antioxidants, the levels of ROS in plant cell interactions is likely underestimated.