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Title: TOWARDS CHARACTERIZATION OF UNKNOWN PROTEINS ENCODED BY THE MAIZE CHLOROTICDWARF VIRUS GENOME

Author
item CHAOUCH, R - OHIO STATE UNIVERSITY
item Willie, Kristen
item Redinbaugh, Margaret
item HOGENHOUT, S - OHIO STATE UNIVERSITY

Submitted to: American Entomological Institute
Publication Type: Abstract Only
Publication Acceptance Date: 12/7/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Maize chlorotic dwarf waikavirus (MCDV) is a phloem-limited, single-stranded RNA virus that causes vein banding and stunting in maize. MCDV is transmitted to maize (Zea mays L.) by the deltocephaline leafhopper, Graminella nigrifons, in a semi-persistent manner in which virus particles bind to the insect's foregut with help of a viral-encoded helper component. The genome of Ohio MCDV-Ws is 11.8 kb long and encodes a single large protein that is post-translationally cleaved into several smaller functional proteins. While regions of the genome encoding the three coat protein subunits, a protease and replicase are identified. The function has not been assigned to proteins encoded by three separate regions of the genome. To begin characterization of these regions, three cDNA fragments encoding them were cloned into the pTrcHis plasmid. The His-tagged proteins were expressed in Escherichia coli and affinity purified for raising antibodies. The antibodies will be used for the characterization and localization of proteins in Western blot assays and microscopy of infected plant material and leafhoppers.