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Title: ANALYSIS OF MONOCLONAL ANTIBODIES REACTING WITH MOLECULES EXPRESSED ON YO T CELLS

Author
item DAVIS, W - DEPT VET MIC PATH PULLMAN
item HAVERSON, K - U BRISTOL, UK
item SAAHMULLER, A - TUBINGEN, GERMANY
item YANG, H - IAH PIRB LAB WOKING UK
item Lunney, Joan
item HAMILTON, M - DEPT VET MIC PATH PULLMAN
item PESCOVITZ, M - INDIANA U. INDIANAPOLIS

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/25/2001
Publication Date: 7/20/2001
Citation: N/A

Interpretive Summary: Mucosal immunity in many species involves unique immune cell subsets. In the pig there are a substantial number of gamma-delta T (gdT) cells in the intestinal tract. Unlike humans and mice there are significant numbers of these gdT cells in the pig peripheral blood. This manuscript presents data from the Third International Swine Cluster of Differentiation (CD) Workshop. For this Third Swine CD Workshop the reactivity of a panel of 26 test monoclonal antibodies (mAb) against gdT cells was analyzed. After comparing the assay data, clustering analysis and inhibition studies, Two mAbs from the 2nd Swine CD workshop recognized a molecule or molecules expressed on subsets of gd T cells. A new mAb (PPT16) added late to the workshop following a request by the workshop chairs appeared to recognize a determinant expressed on the gd TCR/CD3 molecular complex. Differentiating these gdT cells and determining their role in immunity to disease will be aided by the addition of these newly characterized reagents. These mAb will enable swine researchers to better define the cellular interactions that control disease, vaccine, and inflammatory responses.

Technical Abstract: Twenty six monoclonal antibodies (mAbs) selected after the first round of analysis in the 3rd International Swine Workshop were grouped with additional mAbs from the 1st and 2nd workshops and mAbs under study for further evaluation. Preparations of peripheral blood leukocytes were used in single and multicolor flow cytometric analyses. Six mAbs did not react with gd T cells. Two were negative. Seven mAbs recognized molecules expressed on gd T cells that were not lineage restricted. One of these from the first workshop (2B11) yielded a pattern of labeling identical to a mAb under study (PGB73A). Ten mAbs were characterized in previous workshops and known to react with the yb TCR or molecules expressed on subsets of gd T cells. One belonged to SWC4, two to SWC5, and one to SWC6. Two mAbs from the 2nd workshop recognized a molecule or molecules expressed on subsets of gb T cells. A new mAb (PPT16) added late to the workshop following a request by the workshop chairs appeared to recognize a determinant expressed on the gb TCR/CD3 molecular complex.