Author
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Cregan, Perry |
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Submitted to: Proceedings of a National Institute of Health Workshop
Publication Type: Abstract Only Publication Acceptance Date: 11/2/2000 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Human forensic scientists have established a set of 13 short tandem repeat (STR) or microsatellite loci for use in personal identification, parentage testing, and other forensic analyses. Microsatellite loci can also be developed for the identification of plant varieties. Relative to human identification, plant variety identification has a number of unique aspects including the genetic structure of some plant varieties that include varieties consisting of a single inbred genotype to those that are composed of a population of outcrossing individuals. In contrast to human forensics, plant variety identification must function over many generations to properly distinguish a plant variety over time. In addition, some plant species are developed from a limited number of "ancestral genotypes" and thus contain low levels of genetic variation. Soybean [Glycine max (L.) Merr.] is an inbreeding species in which a variety generally consists of one inbred genotype. For purposes of Plant Variety Protection soybean varieties have traditionally been identified by standard pigmentation and morphological traits. We identified a set of 13 loci, from 12 different linkage groups, that easily produced unique microsatellite allele size profiles for each of 66 elite N. American soybean varieties. This set of 13 loci was used to characterize four independent sets of elite varieties that were selected based upon identical maturity, morphological, and pigmentation traits. Based upon these analyses, all varieties could be successfully distinguished using the set of 13 selected microsatellite loci. This set of loci is proposed as a standard set for use in DNA profiling of soybean varieties for purposes of obtaining Plant Variety Protection. |
