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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #119169
Title: IN VIVO BALANCE BETWEEN SYSTEMIC AND LOCAL FACTORS DETERMINES DISCRETE BONE CELL LINEAGE PATTERNS

Author
item BECKMAN, M - VA COMMONWEALTH, RICHMOND
item BJORLING, D - UNIV WI, MADISON, WI
item Horst, Ronald

Submitted to: American Society for Bone and Mineral Research
Publication Type: Abstract Only
Publication Acceptance Date: 9/23/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Multi-gene transcriptional analysis was performed to examine nutritional and in vivo interactions between known systemic modulators of bone cell physiology and local cytokine mediators of bone cell lineage. The combination of calcium and vitamin D deficiency increased bone marrow adipogenesis. In contrast, administration of PTH or 1,25-(OH)2D3 caused hyperplastic bone marrow cellularity. In all groups, osteoclastic activity was assessed by the tartrate-resistant alkaline phosphatase (TRAP) procedure. In a second protocol we examined the early effects of estrogen deficiency and estrogen replacement on bone marrow cytokine profiles using real-time RT-PCR and immunocytochemistry. Mouse femurs from 8 day post-surgery sham, OVX, OVX plus 17-beta-estradiol, and OXV plus progesterone and 17-beta-estradiol were processed for both immunocytochemistry and RNA isolation. In both studies, quantitative RT- PCR analysis of RANK, RANK-ligand, OPG, Cbfa-1, PPAR-gamma, BMP-2, BMP-6, TNF-alpha and IL-1-alpha was performed and the transcriptional patterns of these factors were assessed. Overall, multi-gene regulation of bone marrow cytokines demonstrated predictable in vivo profiles that correlated with cell lineage for adipocytes, osteoblasts, or osteoclasts.