Author
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Dobrinsky, John |
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Submitted to: Theriogenology
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 6/1/2001 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Embryos increase the efficiency of transmitting improved genetic potential and provides: transport of maternal germplasm, rapid line regeneration, increase selection pressure, rescue of health stock from diseased herds, improve quarantine conditions and provide a method for international transport of breeding stock. High costs, disease transmission, health testing and transport regulations of live animals could be reduced by usin embryos. This paper introduces embryo preservation and its use in production and reviews state of the art embryo cryopreservation research. While research on swine embryo preservation has been ongoing for 30 years, most progress in cryopreservation research has occurred since 1995. We have shown developmental competence of cytoskeletal stabilized and vitrified/warmed embryos after transfer to recipient females which farrowed the first reported live offspring from this technology. Further, we discuss unprecedented development of non-invasive methodology to cryopreserve zona pellucida intact morula/early blastocyst stage pig embryos. This novel methodology produced high rates of the first live offspring from non-micromanipulated and cryopreserved morula stage pig embryos with intact zona pellucida. After transfer, vitrified zona pellucida removed embryos can produce live, healthy piglets that grow normally and when mature are excellent fecundity. This technology provides new production alternatives and will revolutionize swine production. |
