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Title: MAIZE GENOMICS: POTENTIAL AND UTILITY OF OAT-MAIZE RADIATION HYBRIDS

Author
item KYNAST, RALF - UNIVERSITY OF MINNESOTA
item OKAGAKI, RON - UNIVERSITY OF MINNESOTA
item RUSSELL, CHARLES - UNIVERSITY OF MINNESOTA
item STEC, ADRIAN - UNIVERSITY OF MINNESOTA
item LIVINGSTON, SUZANNE - UNIVERSITY OF MINNESOTA
item ZAIA, HERIKA - UNIVERSITY OF MINNESOTA
item ODLAND, WADE - UNIVERSITY OF MINNESOTA
item RINES, HOWARD
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 2/20/2001
Publication Date: 2/20/2001
Citation: KYNAST, R.G., OKAGAKI, R.J., RUSSELL, C.D., STEC, A.O., LIVINGSTON, S.M., ZAIA, H.A., ODLAND, W.E., RINES, H.W., PHILLIPS, R.L. MAIZE GENOMICS: POTENTIAL AND UTILITY OF OAT-MAIZE RADIATION HYBRIDS. PLANT AND ANIMAL GENOME IX CONFERENCE. 2001. ABSTRACT. P. 177.

Interpretive Summary:

Technical Abstract: RH (Radiation Hybrid) lines possess radiation-generated chromosome fragments either added or translocated to an alien genome. Oat-maize RH lines can be generated from oat lines with maize chromosome additions by gamma-irradiating monosomic seed and selecting among their offspring for plants that possess fragments of the maize chromosome. We produced a panel of 46 oat-maize RH lines for maize chromosome 9. The identified fragments and their overlaps define map segments with a size <5 Mbp. At present, similar panels in the oat background are being developed for maize chromosomes 2 and 4, plants from irradiated seed are growing to develop RH lines for chromosomes 3 and 6, and monosomic seed are being generated for chromosomes 7 and 1. The RH lines are defined by presence/absence of maize- specific markers. This assay can be automated for high sample-throughput. Oat-maize addition plants have been recovered for each of the 10 maize chromosomes. Genomic DNA of all 10 additions is available for distribution Seed is available for maize chromosome 1, 2, 3, 4, 6, 7, 8, and 9 additions. Besides the development of RH lines for a physical map of maize, our complete oat-maize addition set is also used as a source for maize chromosome sorting and construction of chromosome-specific DNA libraries. Such lines also will allow the study of intergenomic gene expression. Further applications such as analyses of multi-gene families and gene duplications in the maize genome emphasize the utility of the oat-maize addition and RH lines. Research supported under NSF Grant No. 9872650.