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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #119822
Title: MAPPING THE MAIZE GENOME VIA ADDITION LINES AND RADIATION HYBRIDS

Author
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard
item KYNAST, RALF - UNIVERSITY OF MINNESOTA
item OKAGAKI, RON - UNIVERSITY OF MINNESOTA
item ODLAND, WADE - UNIVERSITY OF MINNESOTA

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/17/2001
Publication Date: 1/17/2001
Citation: PHILLIPS, R.L., RINES, H.W., KYNAST, R.G., OKAGAKI, R.J., ODLAND, W.E. MAPPING THE MAIZE GENOME VIA ADDITION LINES AND RADIATION HYBRIDS. PLANT AND ANIMAL GENOME IX CONFERENCE. 2001. ABSTRACT. P. 49.

Interpretive Summary:

Technical Abstract: Mapping and analyzing large genomes requires highly efficient mapping systems. We are developing such a system for the physical mapping of maize sequences based on individual maize segments or chromosome(s) added to an oat genome. This system does not require polymorphisms and can be highly automated. After oat x maize crosses, embryo rescue following chromosome elimination results in the production of haploid oat-maize addition lines. Meiotic restitution in the haploid oat plants carrying a maize chromosome(s) results in viable seed and recovery of disomic addition lines. We now have DNA available from the complete set of 10 addition lines. Seed is available for seven disomic additions (maize chromosomes 1, 2, 3, 4, 6, 7, and 9) and one monosomic addition (chromosome 8). No seed transmission has occurred yet for chromosome 5 and 10 additions. Gamma irradiation of monosomic addition lines allows generation of oat plants containing maize chromosome segment(s), called radiation hybrids (RHs). A radiation hybrid panel for chromosome 9 (46 RH lines) allows mapping to an average <5 Mbp interval. Similar panels are being developed for chromosome 2 (72 candidate lines), chromosome 4 (34 candidate lines), chromosome 6 (progeny of irradiated additions being grown), and chromosome 3 (seed on irradiated plants available). System uses include mapping single sequences to chromosome (addition lines) or physical segment (RHs), mapping multigene families to specific chromosomes (addition lines), cloning member(s) of multigene families from individual chromosomes, cloning of repeated sequences from specific chromosomes, and locating duplicated sequences. Research supported by NSF Grant No.9872650.