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Title: OAT-MAIZE CHROMOSOME MANIPULATION FOR THE PHYSICAL MAPPING OF MAIZE SEQUENCES

Author
item KYNAST, R - UNIVERSITY OF MINNESOTA
item OKAGAKI, R - UNIVERSITY OF MINNESOTA
item ODLAND, W - UNIVERSITY OF MINNESOTA
item STEC, A - UNIVERSITY OF MINNESOTA
item RUSSELL, C - UNIVERSITY OF MINNESOTA
item ZAIA, H - UNIVERSITY OF MINNESOTA
item LIVINGSTON, S - UNIVERSITY OF MINNESOTA
item RINES, HOWARD
item PHILLIPS, R - UNIVERSITY OF MINNESOTA

Submitted to: Maize Genetics Cooperation Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: 3/25/2001
Publication Date: 5/15/2001
Citation: KYNAST, R.G., OKAGAKI, R.J., ODLAND, W.E., STEC, A., RUSSELL, C.D., ZAIA, H., LIVINGSTON, S.M., RINES, H.W., PHILLIPS, R.L. OAT-MAIZE CHROMOSOME MANIPULATION FOR THE PHYSICAL MAPPING OF MAIZE SEQUENCES. MAIZE GENETICS COOPERATION NEWS LETTER. 2001. V. 75. P. 54-55.

Interpretive Summary:

Technical Abstract: We have developed a complete set of oat-maize chromosome additions (OMAs) to map maize sequences and study expression of maize genes in the genetic background of oats. A total of 37 monosomic and disomic addition lines that involve 5 oat (chromosome recipient) and 3 maize (chromosome donor) lines were identified. Fertile lines include a total of 30 single disomic additions (2n = 6x+2 = 44) for maize chromosomes 1, 2, 3, 4, 6, 7, 8, and 9, and a single monosomic addition (2n = 6x+1 = 43) for maize chromosome 8. Four original haploid additions are maintained as tiller-clones because in those recovered haploid monosomic additions (n = 3x+1 = 22) the added maize chromosome did not transmit to the F2 offspring. We evaluated the practicability of OMAs for physical mapping with 50 molecular markers that had been previously placed on linkage maps by Southern hybridization. Forty-eight markers mapped to their expected chromosome. Mapping at high resolution is possible with oat-maize radiation hybrids (RHs) through the use of panels of lines created by radiation-induced breakage. These lines contain chromosome rearrangements and sub-chromosomal segments of maize DNA. Two types of panels are being assembled for future mapping with RHs for maize chromosome 9. The first is a high-resolution panel with more than 40 lines that can give a mapping resolution of approximately 5 Mbp. The second panel is a low-resolution panel of five lines that have overlapping segments and can allocate sequences to discrete locations on the chromosome. The use of OMA and RH lines in conjunction with high throughput technologies can provide an efficient and reliable means to map thousands of maize sequences to chromosome and discrete segments within a chromosome. This work is supported under NSF Grant No. 9872650.