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Title: PHYSICAL MAPPING TO MAIZE CHROMOSOME BY USING A COMPLETE SET OF OAT-MAIZE ADDITION LINES

Author
item KYNAST, R - UNIVERSITY OF MINNESOTA
item ODLAND, W - UNIVERSITY OF MINNESOTA
item OKAGAKI, R - UNIVERSITY OF MINNESOTA
item RUSSELL, C - UNIVERSITY OF MINNESOTA
item STEC, A - UNIVERSITY OF MINNESOTA
item ZAIA, H - UNIVERSITY OF MINNESOTA
item LIVINGSTON, S - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, R - UNIVERSITY OF MINNESOTA

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/25/2001
Publication Date: 5/15/2001
Citation: KYNAST, R.G., ODLAND, W.E., OKAGAKI, R.J., RUSSELL, C.D., STEC, A.O., ZAIA, H.A., LIVINGSTON, S.M., RINES, H.W., PHILLIPS, R.L. PHYSICAL MAPPING TO MAIZE CHROMOSOME BY USING A COMPLETE SET OF OAT-MAIZE ADDITION LINES. 43RD MAIZE GENETICS CONFERENCE. 2001. ABSTRACT. P. 17.

Interpretive Summary:

Technical Abstract: All ten different maize chromosomes were recovered as single additions to haploid oat among 139 maize-positive F1 plants generated from about 60,000 oat x maize crosses. Doubled haploid addition plants (2n = 6x+2 = 44) were produced among the F2 offspring from F1 plants either naturally by meiotic restitution or artificially by colchicine application for maize chromosomes s1, 2, 3, 4, 5, 6, 7, 8, and 9. To date a total of 33 fertile addition line are maintained to produce seed with seven lines each for chromosome 2 and 4, six lines for chromosome 9, four lines for chromosome 7, two lines each for chromosome 3, 5, 6, and 8, and one line for chromosome 1. Maize chromosome 10 has been recovered as an addition to a haploid oat and is vegetatively maintained in the form of tiller-clones; because F2 offspring have not yet been produced, seed is not available. However, genomic DNA from the chromosome 10 addition plant is available to the scientific community. Here we report on the specific characters of these additions an demonstrate their usefulness for mapping genes and markers to chromosome, in particular for high throughput applications like microarray technology. Microarray slides immobilize unlabeled DNA probes to glass, which allows hybridization with labeled DNA. We are optimizing this technology to use labeled genomic DNA from our addition lines to allocate sequences to chromosome(s). This material is based upon work supported by the National Science Foundation under Grant No. 9872650.