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ARS Home » Research » Publications at this Location » Publication #121824
Title: CHARACTERIZATION OF TRINUCLEOTIDE SSR MOTIFS IN WHEAT

Author
item SONG, QIJIANG - VISITING SCIENTIST
item CREGAN, PERRY

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: As compared to dinucleotide-based microsatellite or SSR (simple sequence repeat) markers, polymorphism among trinucleotide-based microsatellite alleles can be more easily detected and frequently produce fewer "stutter bands". Our objectives were to determine the abundance of trinucleotide microsatellites in the wheat genome and the rate at which different motifs can be developed into useful markers. Four genomic libraries of 'Chinese Spring' were screened with trinucleotide probes. Of the selected clones, 70, 45, 42, 27, 14, 11, 10, 10, and 9 contained CTT, GGA, TAA, CAA, GGT, CAT, CTA, CGA and CGT repeats respectively, and 39, 30, 16, 3 and 2 contained TAA, CTT, CAA, CAT and GGA microsatellites with 8 or more perfect repeats, respectively. From these data, 30, 27, and 16 PCR primer sets were designed and tested to the TAA, CTT, and CAA microsatellites, respectively. A total of 13(43%), 4(15%), and 2(12.5%) of these primer sets, respectively, resulted in polymorphic markers. Results indicated that ATT microsatellites will provide the most abundant and polymorphic source of microsatellite markers in wheat.