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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Publications at this Location » Publication #122127

Title: AN IMPROVED GENETIC LINKAGE MAP FOR COWPEA (VIGNA UNGUICULATA L.) COMBINING AFLP, RFLP, RAPID AND BIOCHEMICAL MARKERS

Author
item OUEDRAOGO, J - BURKINA FASO
item GOWDA, B - UNIVERSITY OF VIRGINIA
item JEAN, M - CANADA
item CLOSE, T - UNIV OF CA RIVERSIDE
item EHLERS, J - UNIV OF CA RIVERSIDE
item HALL, A - UNIV OF CA RIVERSIDE
item Gillaspie, Athey - Graves
item ROBERTS, P - UNIV OF CA RIVERSIDE
item ISMAIL, A - PHILIPPINES
item BRUENING, G - UNIV OF CA DAVIS

Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/1/2001
Publication Date: 4/1/2002
Citation: Quedraogo, J.T., Gowda, B.S., Jean, M. Close, T.J., Ehlers, J.D., Hall, A.E., Gillaspie, Jr., A.G., Roberts, P.A., Ismail, A.M., Bruening, G. An improved genetic linkage map for cowpea (vigna unguiculatal) combining aelp, relp, rapid and biochemical markers. Genome. 2002. Genome: 45:175-188 (2002).

Interpretive Summary: An improved genetic linkage map was constructed for cowpea based on various nucleic acid markers and biochemical traits to provide breeders and other scientists information on how to better develop cowpea lines with various resistances. This should lead to cowpea cultivars with better resistances to plant viruses, fungi, nematodes, and witch weed. These new cultivars should produce better yields and seed quality and provide the consumer a better and less expensive product.

Technical Abstract: The public's concerns over environmental issues associated with commercial pesticides has stimulated research on biological control of plant pathogens and pests using legumes. The production of semi-tropical legumes at the USDA Plant Genetic Resources Cons. Unit (Griffin, GA) led us to investigate their activity as soil amendments against root-knot nematode, Meloidogyne incognita. Plant tissue of Aeschynomene spp., Canavalia spp., Crotalaria spp., Indigofera spp., Leucaena spp., Rhynchosia spp., Senna spp., Sesbania spp, and Tephrosia spp. were mixed separately at 1, 2, and 5% (w/w) with soil containing 6,000 nematode eggs per kg. Nematicidal activity was determined two months after planting susceptible tomato. The tissues which were most effective in reducing root-knot nematodes were Canavalia ensiformis, Crotalaria retusa, Indigofera hirsuta, I. nummularifolia, I. spicata, I. suffruticosa, I. tinctoria, and Tephrosia adunca. Special-purpose legumes may hold promise as "natural value added" compounds or as green manure crops for nematode control.