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Title: SUPERCRITICAL FLUID EXTRACTION AND CHROMATOGRAPHY FOR FAT-SOLUBLE VITAMIN ANALYSIS

Author
item TURNER, CHARLOTTA - LUND UNIVERSITY
item King, Jerry
item MATHIASSON, LENNART - LUND UNIVERSITY

Submitted to: Journal of Chromatography
Publication Type: Review Article
Publication Acceptance Date: 10/26/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Extraction and chromatographic separation of fat-soluble vitamins is a challenging task, due to the sensitivity of these compounds towards light, oxygen, heat and pH. In light of this, supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) are attractive techniques as they function at considerably milder conditions than conventional solvent-based analytical techniques. Moreover, supercritical techniques consume much smaller amounts of organic solvents than conventional ones. This review gives a brief description of suitable supercritical media as well as basic theory on SFE and SFC processes. Furthermore, guidelines are provided for optimizing the important extraction and separation parameters to facilitate a successful method development. Finally, applications employing SFE and/or SFC for fat-soluble vitamin enrichment and final determination are reviewed.