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ARS Home » Research » Publications at this Location » Publication #122508
Title: PRESSURIZED HOT WATER EXTRACTION OF PESTICIDES AND ANTIBIOTICS FROM TISSUE SAMPLES DISPERSED WITH AN ACRYLIC POLYMER

Author
item Curren, Meredith
item King, Jerry

Submitted to: Association Official Analytical Chemists Annual Intrl Meeting & Exposition
Publication Type: Abstract Only
Publication Acceptance Date: 9/12/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Pressurized water extraction at elevated temperatures (up to 100 C) has been utilized for the isolation of semi-polar and polar food contaminants from kidney samples. The advantages of employing pressurized hot water as an extraction agent are its non-toxic nature and low cost, and the fact that it can be easily obtained and disposed of. In addition, the sample does not need to be dried prior to the extraction step. The solvent strength of water can be varied with the adjustment of temperature and/or by the use of an organic solvent modifier. In the past, the inherent difficulty in the extraction of meat samples has been in the co-extraction of matrix components such as lipids and proteins. Clean-up of kidney samples has been achieved in situ with the technique of matrix solid-phase dispersion utilizing a methacrylate polymer. The developed method has been applied to the extraction of the pesticide atrazine from beef kidney in addition to the antibiotic avoparcin from swine kidney. The experimental results will be discussed with respect to the extraction temperature, the amount of a non-toxic modifier, the volume of solvent, and optimization of the extraction time. The amount of analyte in the aqueous extracts was quantified by gas chromatography/mass spectrometry or liquid chromatography.