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ARS Home » Research » Publications at this Location » Publication #123531
Title: ELUTION BEHAVIOR OF UNSAPONIFIABLE LIPIDS ON VARIOUS CEC STATIONARY PHASES

Author
item Abidi, Sharon
item THIAM, S - LOUISIANA ST U, B. ROUGE
item WARNER, I. - LOUISIANA ST U, B. ROUGE

Submitted to: Journal of Chromatography A
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2001
Publication Date: 11/20/2002
Citation: ABIDI, S.L., THIAM, S., WARNER, I.M. ELUTION BEHAVIOR OF UNSAPONIFIABLE LIPIDS ON VARIOUS CEC STATIONARY PHASES. JOURNAL OF CHROMATOGRAPHY A. 2002. v. 949. p. 195-207.

Interpretive Summary: Vitamin E-active compounds and phytosterols are important neutraceuticals present as minor constituents in oilseeds. They are useful in oil preservation. Reliable techniques are needed for studying the impact of the minor constituents on the quality and stability of vegetable oils. Migration properties of the cholesterol-lowing substances in edible oils were evaluated by micro-scale high-speed capillary electrochromatography. Rice bran oils and soybean oils were quantified for the antioxidant levels correlatable with frying oil stability. The technique can be used by oil industries for information on minor component compositional factors affecting oil quality/stability and provides useful means to identify component structures for the stabilization of frying oils.

Technical Abstract: Capillary electrochromatographic (CEC)separations of unsaponifiable lipids, tocopherols (T), tocotrienols (T3), and plant sterols were studied under various conditions. Investigated stationary phases include pentafluorophenylsilica (PFPS), triacontylsilica (TCS), and octadecylsilica (ODS) phases. A baseline separation of four sterols on ODS was achieved. CEC of the toco derived compounds on PFPS in aqueous methanol yielded the most satisfactory results with complete resolution of all components while a reversal in elution of one pair was observed in aqueous acetonitrile. CEC with a TCS phase in nonaqueous methanol led to a different elution pattern despite favorable resolution one pair along with the observation of inseparable pairs in nonaqueous dimethyl formamide. Variations in resolution and retention factors of relevant antioxidant species with CEC variables were evaluated. Examples of CEC quantification of unsaponifiable fractions of rice bran oils and soybean oils used in frying potato chips are presented.