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ARS Home » Pacific West Area » Logan, Utah » Poisonous Plant Research » Research » Publications at this Location » Publication #124360
Title: DEVELOPMENT OF STS AND CAPS MARKERS FOR IDENTIFICATION THREE TALL LARKSPURS (DELPHINIUM SPP.)

Author
item LI, XIAOMEI
item Gardner, Dale
item Ralphs, Michael
item Wang, Richard

Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/22/2001
Publication Date: 2/7/2002
Citation: LI, X., GARDNER, D.R., RALPHS, M.H., WANG, R. DEVELOPMENT OF STS AND CAPS MARKERS FOR IDENTIFICATION THREE TALL LARKSPURS (DELPHINIUM SPP.). GENOME. 2002.

Interpretive Summary: Some toxic tall larkspur (Delphinium spp.) plants were classified into three species based on the sequence of previously identified species- specific random amplified DNA markers. Four markers were used for Delphinium occidentale identification, three markers for D. barbeyi, and three markers for D. glaucum. One hundred sixty-six individual plants, collected at 19 locations in the western U.S., were tested. Over 95% of the D. occidentale plants contained all four occidentale-specific markers, while the remaining plants contained three of the four markers. Approximately 97% of D. barbeyi plants contained all three barbeyi-specific markers, and the rest had two of the three markers. A small percentage of D. barbeyi plants contained one occidentale-specific marker. Hybrid populations were characterized in having more occidentale-specific markers than barbeyi-specific markers. This set of molecular based markers for larkspur species should be useful in classification of unknown plant materials and the identification of hybrid populations.

Technical Abstract: One cleaved amplified polymorphic sequence (CAPS) and nine sequence tagged site (STS) markers were developed for classifying tall larkspur (Delphinium spp.) plants in three species based on the DNA sequence of known species- specific RAPD markers. Four STS markers were used for Delphinium occidentale identification, three STS markers for D. Barbeyi, and one CAPS and two STS markers for D. glaucum. One hundred sixty-six individual plants, collected at 19 locations in western U.S., were tested using the STS and CAPS markers. Over 95% of the D. occidentale plants contained all four occidentale-specific STS markers, while the remaining plants contained three of the four STS markers. Approximately 97% of D. barbeyi plants contained all three barbeyi-specific STS markers and the rest had two of the three STS markers. A small percentage of D. barbeyi plants contained one occidentale-specific STS marker. Hybrid populations were characterized din having more occidentale-specific STS markers than barbeyi-specific STS markers, suggesting that the three hybrid populations are composed of not F1 hybrid plants of the parental species but segregating offsprings of different generations from original hybrids. This set of STS and CAPS markers for larkspur species should be useful in classification of unknown plant materials and the identification of hybrid populations.