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ARS Home » Research » Publications at this Location » Publication #125207
Title: GALLUS GALLUS MALIC ENZYME, NADP (+)-DEPENDENT, CYSTOSOLIC, MRNA, COMPLETE CDS

Author
item Poch, Stephen
item Richards, Mark

Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 9/2/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A portion of the cytosolic NADP+-dependent malate dehydrogenase (EC1.1.1.40, malic enzyme) gene was isolated, sequenced and characterized from chicken liver tissue using reverse transcription polymerase chain reaction (RT-PCR). Direct sequencing of overlapping PCR-generated DNA fragments was performed to identify a total of 1,982 contiguous bases corresponding to the malic enzyme mRNA (cDNA) and including the complete coding region, as well as the 5'- and 3'-untranslated regions. The resulting nucleotide sequence showed identities of 92, 91, 83, 81 and 80% with corresponding sequences previously reported for duck, pigeon, human, mouse and pig, respectively. From the nucleotide sequence, a protein consisting of 557 amino acids was predicted that showed homology of 97, 96, 89, 89 and 88% with corresponding amino acid sequence data previously reported for duck, pigeon, human, pig, and mouse, respectively. The predicted amino acid sequence demonstrated complete conservation of lysine (positions 162 and 340) and aspartic acid residues (positions 141 and 194) thought to be involved at the enzyme's active site, as well as an aspartic acid residue (position 324) that is conserved in all malic enzymes. Further, a specific dinucleotide-binding signature motif was identified at positions 289-294. This sequence data will be useful in the study of the structure and function of malic enzymes and in determining the role of expression of this gene in the regulation of lipogenesis in chickens.